Can we correlate shape / form with function?
How does the virus bind to the cell?
How do virus capsid mechanical properties relate to infectivity?
Showing shape with a 3D rendering reinforced by a color map from shape -
different slopes are different colors. PS sphere is a control, to show it wasn't
because of a triangular tip.
Using directional illumination to show the shape of a virus, highlighting the
facets.
Translating a virus in solution
Quantify the lateral force required to move a virus across the silicon
substrate in solution.
An adenovirus before and after indenting with an AFM tip. This indentation
was performed in air.
Huge difference in the force required to indent a virus in water and in air.
PS beads shown as a control.
More details can be found in these references:
Guthold, M., Matthews, G., Negishi, A., Taylor, R. M., Erie, D., Brooks, F.
P. & Superfine, R. (1999). Quantitative Manipulation of DNA and Viruses with
the nanoManipulator Scanning Force Microscope. Surf. Interf. Analys. 27,
437-443
Guthold, M., Falvo, M. R., Matthews, W. G., Paulson, S., Washburn, S., Erie, D., Superfine, R., Brooks,
F. P. & Taylor, R. M. (2000). Controlled Manipulation of Molecular Samples with the
nanoManipulator.IEEE/ASME Transactions on Mechatronics. 5, 189-198
Guthold, M., Falvo, M., Matthews, W. G., Paulson, S., Mullin, J., Lord, S., Erie, D., Washburn, S.,
Superfine, R., Brooks, F. P. & Taylor, R. M. (1999). Investigation and Modification of Molecular Structures Using the NanoManipulator. J Mol. Graphics Mod. 17,
187-197
"Manipulation of Individual Viruses: Friction and Mechanical Properties,"
M. R. Falvo, S. Washburn, R. Superfine, M. Finch, F. P. Brooks, Jr., V. Chi,
and R. M. Taylor II, Biophysical Journal ,72, 1396-1403. (1997)
Matthews, W. G., Negishi, A., Seeger, A., Taylor, R., McCarty, D. M.,
Samulski, R. J. and Superfine, R. (1999). "Elasticity and binding of
adenovirus in air and in liquid." Biophysical Society 43rd annual
meeting, Feb. 13-17, 1999, Baltimore, MD; Biophys. J. 76, A265.
Negishi, A., Matthews, W. G., McCarty, D. M., Samulski, R. J., Rohrer, D.,
Henderson, A., Taylor, R. and Superfine, R. (1999). "Probing the structural
properties of adenovirus using the atomic force microscope." Biophysical
Society 43rd annual meeting, Feb. 13-17, 1999, Baltimore, MD; Biophys. J.
76, A27.
Images and scientific content are courtesy of the University of North Carolina at Chapel Hill.
